human embryonic stem cell marker antibody panel Search Results


mhc emyhc  (Developmental Studies Hybridoma Bank)
99
Developmental Studies Hybridoma Bank mhc emyhc
Analysis of regeneration and necrosis in quadriceps of mdx mice. (A) Muscle sections of quadriceps from mdx mice treated with AAV8-tMCK-A20 (AAV8-A20) or saline were labeled with embryonic myosin heavy chain <t>(eMyHC)</t> antibody (red) or IgG (green) to assess the number of regenerating fibers and necrotic fibers, respectively. Quantification of the percentage of regenerating (B) and necrotic (C) fibers is shown. n = 9 for saline-treated mdx mice; n = 12 for AAV8-tMCK-A20–treated mdx mice. Scale bar = 100 μm; *p < 0.05.
Mhc Emyhc, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti human mouse ssea 1  (R&D Systems)
92
R&D Systems anti human mouse ssea 1
Expression of pluripotency markers in mDFAT cells. (A): Expression of Oct3/4, SOX2, Nanog, c-Myc, and Klf4, pluripotency markers, and c-Kit, a mesenchymal stem cell marker, was detected in mDFAT cells by reverse transcription polymerase chain reaction (PCR) on day 3. Isolated mouse adipocytes and mouse embryonic stem cells were used as negative and positive controls, respectively, and mouse adipose stromal cells are shown for comparison. GAPDH was used as RNA control. (B): Expression of Oct3/4, SOX2, Nanog, and c-Kit decreased in mDFAT cells between day 3 and day 10, whereas expression of Sca1, a mesenchymal stem cell marker, and BMP4, a stem cell renewal factor, increased after 3 days, as determined by real-time PCR. Asterisks indicate a statistically significant difference as compared with day 0: ∗, p < .05; ∗, p <. 01; ∗∗∗, p < .001; Tukey’s test. (C): Immunolocalization of Nanog, Oct3/4, Tra-1, Sca1, c-Kit, and BMP4 in single mDFAT cells on day 0. DAPI (blue) was used to visualize nuclei. (D): Characteristic mDFAT cell clusters formed spontaneously in mDFAT cell cultures on day 3 and later, as visualized by reverse-phase microscope (bright field). (E): Immunolocalization <t>of</t> <t>SSEA-1,</t> SSEA-3, SOX2, Oct3/4, c-Myc, and Klf4 in cell clusters on days 5–7. Abbreviations: BF, bright field; DAPI, 4′,6-diamidino-2-phenylindole; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; mAdipo, mouse adipocytes; mASC, mouse adipose stromal cells; mDFAT, mouse dedifferentiated fat; mESC, mouse embryonic stem cells; SOX2, sex-determining region Y-box 2; SSEA, stage-specific embryonic antigen.
Anti Human Mouse Ssea 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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stem cell research 49 2020 102094 pluripotency markers flow cytometry oct 4a rabbit mab  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc stem cell research 49 2020 102094 pluripotency markers flow cytometry oct 4a rabbit mab
Expression of pluripotency markers in mDFAT cells. (A): Expression of Oct3/4, SOX2, Nanog, c-Myc, and Klf4, pluripotency markers, and c-Kit, a mesenchymal stem cell marker, was detected in mDFAT cells by reverse transcription polymerase chain reaction (PCR) on day 3. Isolated mouse adipocytes and mouse embryonic stem cells were used as negative and positive controls, respectively, and mouse adipose stromal cells are shown for comparison. GAPDH was used as RNA control. (B): Expression of Oct3/4, SOX2, Nanog, and c-Kit decreased in mDFAT cells between day 3 and day 10, whereas expression of Sca1, a mesenchymal stem cell marker, and BMP4, a stem cell renewal factor, increased after 3 days, as determined by real-time PCR. Asterisks indicate a statistically significant difference as compared with day 0: ∗, p < .05; ∗, p <. 01; ∗∗∗, p < .001; Tukey’s test. (C): Immunolocalization of Nanog, Oct3/4, Tra-1, Sca1, c-Kit, and BMP4 in single mDFAT cells on day 0. DAPI (blue) was used to visualize nuclei. (D): Characteristic mDFAT cell clusters formed spontaneously in mDFAT cell cultures on day 3 and later, as visualized by reverse-phase microscope (bright field). (E): Immunolocalization <t>of</t> <t>SSEA-1,</t> SSEA-3, SOX2, Oct3/4, c-Myc, and Klf4 in cell clusters on days 5–7. Abbreviations: BF, bright field; DAPI, 4′,6-diamidino-2-phenylindole; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; mAdipo, mouse adipocytes; mASC, mouse adipose stromal cells; mDFAT, mouse dedifferentiated fat; mESC, mouse embryonic stem cells; SOX2, sex-determining region Y-box 2; SSEA, stage-specific embryonic antigen.
Stem Cell Research 49 2020 102094 Pluripotency Markers Flow Cytometry Oct 4a Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ssea 4  (Santa Cruz Biotechnology)
95
Santa Cruz Biotechnology ssea 4
Expression of pluripotency markers in mDFAT cells. (A): Expression of Oct3/4, SOX2, Nanog, c-Myc, and Klf4, pluripotency markers, and c-Kit, a mesenchymal stem cell marker, was detected in mDFAT cells by reverse transcription polymerase chain reaction (PCR) on day 3. Isolated mouse adipocytes and mouse embryonic stem cells were used as negative and positive controls, respectively, and mouse adipose stromal cells are shown for comparison. GAPDH was used as RNA control. (B): Expression of Oct3/4, SOX2, Nanog, and c-Kit decreased in mDFAT cells between day 3 and day 10, whereas expression of Sca1, a mesenchymal stem cell marker, and BMP4, a stem cell renewal factor, increased after 3 days, as determined by real-time PCR. Asterisks indicate a statistically significant difference as compared with day 0: ∗, p < .05; ∗, p <. 01; ∗∗∗, p < .001; Tukey’s test. (C): Immunolocalization of Nanog, Oct3/4, Tra-1, Sca1, c-Kit, and BMP4 in single mDFAT cells on day 0. DAPI (blue) was used to visualize nuclei. (D): Characteristic mDFAT cell clusters formed spontaneously in mDFAT cell cultures on day 3 and later, as visualized by reverse-phase microscope (bright field). (E): Immunolocalization <t>of</t> <t>SSEA-1,</t> SSEA-3, SOX2, Oct3/4, c-Myc, and Klf4 in cell clusters on days 5–7. Abbreviations: BF, bright field; DAPI, 4′,6-diamidino-2-phenylindole; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; mAdipo, mouse adipocytes; mASC, mouse adipose stromal cells; mDFAT, mouse dedifferentiated fat; mESC, mouse embryonic stem cells; SOX2, sex-determining region Y-box 2; SSEA, stage-specific embryonic antigen.
Ssea 4, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti ssea 1  (R&D Systems)
92
R&D Systems anti ssea 1
Expression of pluripotency markers in mDFAT cells. (A): Expression of Oct3/4, SOX2, Nanog, c-Myc, and Klf4, pluripotency markers, and c-Kit, a mesenchymal stem cell marker, was detected in mDFAT cells by reverse transcription polymerase chain reaction (PCR) on day 3. Isolated mouse adipocytes and mouse embryonic stem cells were used as negative and positive controls, respectively, and mouse adipose stromal cells are shown for comparison. GAPDH was used as RNA control. (B): Expression of Oct3/4, SOX2, Nanog, and c-Kit decreased in mDFAT cells between day 3 and day 10, whereas expression of Sca1, a mesenchymal stem cell marker, and BMP4, a stem cell renewal factor, increased after 3 days, as determined by real-time PCR. Asterisks indicate a statistically significant difference as compared with day 0: ∗, p < .05; ∗, p <. 01; ∗∗∗, p < .001; Tukey’s test. (C): Immunolocalization of Nanog, Oct3/4, Tra-1, Sca1, c-Kit, and BMP4 in single mDFAT cells on day 0. DAPI (blue) was used to visualize nuclei. (D): Characteristic mDFAT cell clusters formed spontaneously in mDFAT cell cultures on day 3 and later, as visualized by reverse-phase microscope (bright field). (E): Immunolocalization <t>of</t> <t>SSEA-1,</t> SSEA-3, SOX2, Oct3/4, c-Myc, and Klf4 in cell clusters on days 5–7. Abbreviations: BF, bright field; DAPI, 4′,6-diamidino-2-phenylindole; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; mAdipo, mouse adipocytes; mASC, mouse adipose stromal cells; mDFAT, mouse dedifferentiated fat; mESC, mouse embryonic stem cells; SOX2, sex-determining region Y-box 2; SSEA, stage-specific embryonic antigen.
Anti Ssea 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human embryonic stem cell marker antibody panel  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology human embryonic stem cell marker antibody panel
Expression of pluripotency markers in mDFAT cells. (A): Expression of Oct3/4, SOX2, Nanog, c-Myc, and Klf4, pluripotency markers, and c-Kit, a mesenchymal stem cell marker, was detected in mDFAT cells by reverse transcription polymerase chain reaction (PCR) on day 3. Isolated mouse adipocytes and mouse embryonic stem cells were used as negative and positive controls, respectively, and mouse adipose stromal cells are shown for comparison. GAPDH was used as RNA control. (B): Expression of Oct3/4, SOX2, Nanog, and c-Kit decreased in mDFAT cells between day 3 and day 10, whereas expression of Sca1, a mesenchymal stem cell marker, and BMP4, a stem cell renewal factor, increased after 3 days, as determined by real-time PCR. Asterisks indicate a statistically significant difference as compared with day 0: ∗, p < .05; ∗, p <. 01; ∗∗∗, p < .001; Tukey’s test. (C): Immunolocalization of Nanog, Oct3/4, Tra-1, Sca1, c-Kit, and BMP4 in single mDFAT cells on day 0. DAPI (blue) was used to visualize nuclei. (D): Characteristic mDFAT cell clusters formed spontaneously in mDFAT cell cultures on day 3 and later, as visualized by reverse-phase microscope (bright field). (E): Immunolocalization <t>of</t> <t>SSEA-1,</t> SSEA-3, SOX2, Oct3/4, c-Myc, and Klf4 in cell clusters on days 5–7. Abbreviations: BF, bright field; DAPI, 4′,6-diamidino-2-phenylindole; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; mAdipo, mouse adipocytes; mASC, mouse adipose stromal cells; mDFAT, mouse dedifferentiated fat; mESC, mouse embryonic stem cells; SOX2, sex-determining region Y-box 2; SSEA, stage-specific embryonic antigen.
Human Embryonic Stem Cell Marker Antibody Panel, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ssea 1  (Santa Cruz Biotechnology)
95
Santa Cruz Biotechnology ssea 1
Expression of pluripotency markers in mDFAT cells. (A): Expression of Oct3/4, SOX2, Nanog, c-Myc, and Klf4, pluripotency markers, and c-Kit, a mesenchymal stem cell marker, was detected in mDFAT cells by reverse transcription polymerase chain reaction (PCR) on day 3. Isolated mouse adipocytes and mouse embryonic stem cells were used as negative and positive controls, respectively, and mouse adipose stromal cells are shown for comparison. GAPDH was used as RNA control. (B): Expression of Oct3/4, SOX2, Nanog, and c-Kit decreased in mDFAT cells between day 3 and day 10, whereas expression of Sca1, a mesenchymal stem cell marker, and BMP4, a stem cell renewal factor, increased after 3 days, as determined by real-time PCR. Asterisks indicate a statistically significant difference as compared with day 0: ∗, p < .05; ∗, p <. 01; ∗∗∗, p < .001; Tukey’s test. (C): Immunolocalization of Nanog, Oct3/4, Tra-1, Sca1, c-Kit, and BMP4 in single mDFAT cells on day 0. DAPI (blue) was used to visualize nuclei. (D): Characteristic mDFAT cell clusters formed spontaneously in mDFAT cell cultures on day 3 and later, as visualized by reverse-phase microscope (bright field). (E): Immunolocalization <t>of</t> <t>SSEA-1,</t> SSEA-3, SOX2, Oct3/4, c-Myc, and Klf4 in cell clusters on days 5–7. Abbreviations: BF, bright field; DAPI, 4′,6-diamidino-2-phenylindole; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; mAdipo, mouse adipocytes; mASC, mouse adipose stromal cells; mDFAT, mouse dedifferentiated fat; mESC, mouse embryonic stem cells; SOX2, sex-determining region Y-box 2; SSEA, stage-specific embryonic antigen.
Ssea 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse anti-human ssea-4-pe  (Millipore)
90
Millipore mouse anti-human ssea-4-pe
Expression of pluripotency markers in mDFAT cells. (A): Expression of Oct3/4, SOX2, Nanog, c-Myc, and Klf4, pluripotency markers, and c-Kit, a mesenchymal stem cell marker, was detected in mDFAT cells by reverse transcription polymerase chain reaction (PCR) on day 3. Isolated mouse adipocytes and mouse embryonic stem cells were used as negative and positive controls, respectively, and mouse adipose stromal cells are shown for comparison. GAPDH was used as RNA control. (B): Expression of Oct3/4, SOX2, Nanog, and c-Kit decreased in mDFAT cells between day 3 and day 10, whereas expression of Sca1, a mesenchymal stem cell marker, and BMP4, a stem cell renewal factor, increased after 3 days, as determined by real-time PCR. Asterisks indicate a statistically significant difference as compared with day 0: ∗, p < .05; ∗, p <. 01; ∗∗∗, p < .001; Tukey’s test. (C): Immunolocalization of Nanog, Oct3/4, Tra-1, Sca1, c-Kit, and BMP4 in single mDFAT cells on day 0. DAPI (blue) was used to visualize nuclei. (D): Characteristic mDFAT cell clusters formed spontaneously in mDFAT cell cultures on day 3 and later, as visualized by reverse-phase microscope (bright field). (E): Immunolocalization <t>of</t> <t>SSEA-1,</t> SSEA-3, SOX2, Oct3/4, c-Myc, and Klf4 in cell clusters on days 5–7. Abbreviations: BF, bright field; DAPI, 4′,6-diamidino-2-phenylindole; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; mAdipo, mouse adipocytes; mASC, mouse adipose stromal cells; mDFAT, mouse dedifferentiated fat; mESC, mouse embryonic stem cells; SOX2, sex-determining region Y-box 2; SSEA, stage-specific embryonic antigen.
Mouse Anti Human Ssea 4 Pe, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti human ubiquitin antibody  (R&D Systems)
93
R&D Systems anti human ubiquitin antibody
Immunological validation of <t>ubiquitin</t> and S100P. ( A ) For ubiquitin, four BC and corresponding AT extracts were analysed by immunoblotting, indicating relative upregulation of ubiquitin in some breast cancer patients. β -Actin is shown as a loading control. ( B ) Densitometric analysis of ubiquitin western blots of eight sample pairs. Box plot shows median and upper and lower quartiles; lines show maximum and minimum values. P =0.017, Wilcoxon signed-rank test. ( C ) Mass spectrometry (MS) spectra of proteins bound to immobilised mouse anti-ubiquitin antibody. Samples were (i) patient 1 normal tissue, (ii) patient 1 cancer tissue, (iii) patient 2 normal tissue, (iv) patient 2 cancer tissue, (v) recombinant His-tagged ubiquitin, and (vi) patient 2 cancer tissue, mouse IgG control. Arrow indicates the mass of monomeric ubiquitin, m/z 8558. N=normal tissue; C=cancer tissue. ( D ) For S100P, four BC and corresponding AT extracts were analysed by immunoblotting, indicating relative upregulation of S100P in some breast cancer patients. β -Actin is shown as a loading control. ( E ) Densitometric analysis of S100P Western blots of 8 sample pairs. Box plot shows median and upper and lower quartiles; lines show maximum and minimum values. P =0.012, Wilcoxon signed-rank test. ( F ) Mass spectrometry spectra of proteins bound to immobilised rabbit anti-S100P antibody. Samples were (i) patient 3 normal tissue, (ii) patient 3 cancer tissue, (iii) patient 4 normal tissue, (iv) patient 4 cancer tissue, (v) recombinant His-tagged S100P, and (vi) patient 4 cancer tissue, rabbit IgG control. Arrow indicates the mass of the S100P form of m/z 9226. N=normal tissue; C=cancer tissue.
Anti Human Ubiquitin Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human embryonic kidney hek 293 cells  (OriGene)
95
OriGene human embryonic kidney hek 293 cells
Immunological validation of <t>ubiquitin</t> and S100P. ( A ) For ubiquitin, four BC and corresponding AT extracts were analysed by immunoblotting, indicating relative upregulation of ubiquitin in some breast cancer patients. β -Actin is shown as a loading control. ( B ) Densitometric analysis of ubiquitin western blots of eight sample pairs. Box plot shows median and upper and lower quartiles; lines show maximum and minimum values. P =0.017, Wilcoxon signed-rank test. ( C ) Mass spectrometry (MS) spectra of proteins bound to immobilised mouse anti-ubiquitin antibody. Samples were (i) patient 1 normal tissue, (ii) patient 1 cancer tissue, (iii) patient 2 normal tissue, (iv) patient 2 cancer tissue, (v) recombinant His-tagged ubiquitin, and (vi) patient 2 cancer tissue, mouse IgG control. Arrow indicates the mass of monomeric ubiquitin, m/z 8558. N=normal tissue; C=cancer tissue. ( D ) For S100P, four BC and corresponding AT extracts were analysed by immunoblotting, indicating relative upregulation of S100P in some breast cancer patients. β -Actin is shown as a loading control. ( E ) Densitometric analysis of S100P Western blots of 8 sample pairs. Box plot shows median and upper and lower quartiles; lines show maximum and minimum values. P =0.012, Wilcoxon signed-rank test. ( F ) Mass spectrometry spectra of proteins bound to immobilised rabbit anti-S100P antibody. Samples were (i) patient 3 normal tissue, (ii) patient 3 cancer tissue, (iii) patient 4 normal tissue, (iv) patient 4 cancer tissue, (v) recombinant His-tagged S100P, and (vi) patient 4 cancer tissue, rabbit IgG control. Arrow indicates the mass of the S100P form of m/z 9226. N=normal tissue; C=cancer tissue.
Human Embryonic Kidney Hek 293 Cells, supplied by OriGene, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sert overexpression 293t cell lysate h00006532-t01  (Novus Biologicals)
90
Novus Biologicals sert overexpression 293t cell lysate h00006532-t01
Immunological validation of <t>ubiquitin</t> and S100P. ( A ) For ubiquitin, four BC and corresponding AT extracts were analysed by immunoblotting, indicating relative upregulation of ubiquitin in some breast cancer patients. β -Actin is shown as a loading control. ( B ) Densitometric analysis of ubiquitin western blots of eight sample pairs. Box plot shows median and upper and lower quartiles; lines show maximum and minimum values. P =0.017, Wilcoxon signed-rank test. ( C ) Mass spectrometry (MS) spectra of proteins bound to immobilised mouse anti-ubiquitin antibody. Samples were (i) patient 1 normal tissue, (ii) patient 1 cancer tissue, (iii) patient 2 normal tissue, (iv) patient 2 cancer tissue, (v) recombinant His-tagged ubiquitin, and (vi) patient 2 cancer tissue, mouse IgG control. Arrow indicates the mass of monomeric ubiquitin, m/z 8558. N=normal tissue; C=cancer tissue. ( D ) For S100P, four BC and corresponding AT extracts were analysed by immunoblotting, indicating relative upregulation of S100P in some breast cancer patients. β -Actin is shown as a loading control. ( E ) Densitometric analysis of S100P Western blots of 8 sample pairs. Box plot shows median and upper and lower quartiles; lines show maximum and minimum values. P =0.012, Wilcoxon signed-rank test. ( F ) Mass spectrometry spectra of proteins bound to immobilised rabbit anti-S100P antibody. Samples were (i) patient 3 normal tissue, (ii) patient 3 cancer tissue, (iii) patient 4 normal tissue, (iv) patient 4 cancer tissue, (v) recombinant His-tagged S100P, and (vi) patient 4 cancer tissue, rabbit IgG control. Arrow indicates the mass of the S100P form of m/z 9226. N=normal tissue; C=cancer tissue.
Sert Overexpression 293t Cell Lysate H00006532 T01, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ssea 3  (Developmental Studies Hybridoma Bank)
94
Developmental Studies Hybridoma Bank ssea 3
Immunological validation of <t>ubiquitin</t> and S100P. ( A ) For ubiquitin, four BC and corresponding AT extracts were analysed by immunoblotting, indicating relative upregulation of ubiquitin in some breast cancer patients. β -Actin is shown as a loading control. ( B ) Densitometric analysis of ubiquitin western blots of eight sample pairs. Box plot shows median and upper and lower quartiles; lines show maximum and minimum values. P =0.017, Wilcoxon signed-rank test. ( C ) Mass spectrometry (MS) spectra of proteins bound to immobilised mouse anti-ubiquitin antibody. Samples were (i) patient 1 normal tissue, (ii) patient 1 cancer tissue, (iii) patient 2 normal tissue, (iv) patient 2 cancer tissue, (v) recombinant His-tagged ubiquitin, and (vi) patient 2 cancer tissue, mouse IgG control. Arrow indicates the mass of monomeric ubiquitin, m/z 8558. N=normal tissue; C=cancer tissue. ( D ) For S100P, four BC and corresponding AT extracts were analysed by immunoblotting, indicating relative upregulation of S100P in some breast cancer patients. β -Actin is shown as a loading control. ( E ) Densitometric analysis of S100P Western blots of 8 sample pairs. Box plot shows median and upper and lower quartiles; lines show maximum and minimum values. P =0.012, Wilcoxon signed-rank test. ( F ) Mass spectrometry spectra of proteins bound to immobilised rabbit anti-S100P antibody. Samples were (i) patient 3 normal tissue, (ii) patient 3 cancer tissue, (iii) patient 4 normal tissue, (iv) patient 4 cancer tissue, (v) recombinant His-tagged S100P, and (vi) patient 4 cancer tissue, rabbit IgG control. Arrow indicates the mass of the S100P form of m/z 9226. N=normal tissue; C=cancer tissue.
Ssea 3, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Analysis of regeneration and necrosis in quadriceps of mdx mice. (A) Muscle sections of quadriceps from mdx mice treated with AAV8-tMCK-A20 (AAV8-A20) or saline were labeled with embryonic myosin heavy chain (eMyHC) antibody (red) or IgG (green) to assess the number of regenerating fibers and necrotic fibers, respectively. Quantification of the percentage of regenerating (B) and necrotic (C) fibers is shown. n = 9 for saline-treated mdx mice; n = 12 for AAV8-tMCK-A20–treated mdx mice. Scale bar = 100 μm; *p < 0.05.

Journal: Molecular Medicine

Article Title: Adeno-associated Virus Serotype 8 (AAV8) Delivery of Recombinant A20 to Skeletal Muscle Reduces Pathological Activation of Nuclear Factor (NF)-?B in Muscle of mdx Mice

doi: 10.2119/molmed.2012.00299

Figure Lengend Snippet: Analysis of regeneration and necrosis in quadriceps of mdx mice. (A) Muscle sections of quadriceps from mdx mice treated with AAV8-tMCK-A20 (AAV8-A20) or saline were labeled with embryonic myosin heavy chain (eMyHC) antibody (red) or IgG (green) to assess the number of regenerating fibers and necrotic fibers, respectively. Quantification of the percentage of regenerating (B) and necrotic (C) fibers is shown. n = 9 for saline-treated mdx mice; n = 12 for AAV8-tMCK-A20–treated mdx mice. Scale bar = 100 μm; *p < 0.05.

Article Snippet: The monoclonal antibody to embryonic MHC (eMyHC) (F1.652), used to detect regenerating fibers, was obtained from the Developmental Studies Hybridoma Bank, developed by Helen Blau (University of Iowa, Department of Biological Sciences, Iowa City, IA, USA).

Techniques: Saline, Labeling

Expression of pluripotency markers in mDFAT cells. (A): Expression of Oct3/4, SOX2, Nanog, c-Myc, and Klf4, pluripotency markers, and c-Kit, a mesenchymal stem cell marker, was detected in mDFAT cells by reverse transcription polymerase chain reaction (PCR) on day 3. Isolated mouse adipocytes and mouse embryonic stem cells were used as negative and positive controls, respectively, and mouse adipose stromal cells are shown for comparison. GAPDH was used as RNA control. (B): Expression of Oct3/4, SOX2, Nanog, and c-Kit decreased in mDFAT cells between day 3 and day 10, whereas expression of Sca1, a mesenchymal stem cell marker, and BMP4, a stem cell renewal factor, increased after 3 days, as determined by real-time PCR. Asterisks indicate a statistically significant difference as compared with day 0: ∗, p < .05; ∗, p <. 01; ∗∗∗, p < .001; Tukey’s test. (C): Immunolocalization of Nanog, Oct3/4, Tra-1, Sca1, c-Kit, and BMP4 in single mDFAT cells on day 0. DAPI (blue) was used to visualize nuclei. (D): Characteristic mDFAT cell clusters formed spontaneously in mDFAT cell cultures on day 3 and later, as visualized by reverse-phase microscope (bright field). (E): Immunolocalization of SSEA-1, SSEA-3, SOX2, Oct3/4, c-Myc, and Klf4 in cell clusters on days 5–7. Abbreviations: BF, bright field; DAPI, 4′,6-diamidino-2-phenylindole; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; mAdipo, mouse adipocytes; mASC, mouse adipose stromal cells; mDFAT, mouse dedifferentiated fat; mESC, mouse embryonic stem cells; SOX2, sex-determining region Y-box 2; SSEA, stage-specific embryonic antigen.

Journal: Stem Cells Translational Medicine

Article Title: Pluripotent Stem Cells Derived From Mouse and Human White Mature Adipocytes

doi: 10.5966/sctm.2013-0107

Figure Lengend Snippet: Expression of pluripotency markers in mDFAT cells. (A): Expression of Oct3/4, SOX2, Nanog, c-Myc, and Klf4, pluripotency markers, and c-Kit, a mesenchymal stem cell marker, was detected in mDFAT cells by reverse transcription polymerase chain reaction (PCR) on day 3. Isolated mouse adipocytes and mouse embryonic stem cells were used as negative and positive controls, respectively, and mouse adipose stromal cells are shown for comparison. GAPDH was used as RNA control. (B): Expression of Oct3/4, SOX2, Nanog, and c-Kit decreased in mDFAT cells between day 3 and day 10, whereas expression of Sca1, a mesenchymal stem cell marker, and BMP4, a stem cell renewal factor, increased after 3 days, as determined by real-time PCR. Asterisks indicate a statistically significant difference as compared with day 0: ∗, p < .05; ∗, p <. 01; ∗∗∗, p < .001; Tukey’s test. (C): Immunolocalization of Nanog, Oct3/4, Tra-1, Sca1, c-Kit, and BMP4 in single mDFAT cells on day 0. DAPI (blue) was used to visualize nuclei. (D): Characteristic mDFAT cell clusters formed spontaneously in mDFAT cell cultures on day 3 and later, as visualized by reverse-phase microscope (bright field). (E): Immunolocalization of SSEA-1, SSEA-3, SOX2, Oct3/4, c-Myc, and Klf4 in cell clusters on days 5–7. Abbreviations: BF, bright field; DAPI, 4′,6-diamidino-2-phenylindole; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; mAdipo, mouse adipocytes; mASC, mouse adipose stromal cells; mDFAT, mouse dedifferentiated fat; mESC, mouse embryonic stem cells; SOX2, sex-determining region Y-box 2; SSEA, stage-specific embryonic antigen.

Article Snippet: We used the following antibodies for immunostaining: rat anti-stage-specific embryonic antigen (SSEA)-3, mouse anti-SSEA-4, rabbit anti-Neurofilament-66, and mouse anti-human mitochondria (all from Millipore/Chemicon, Billerica, MA, http://www.millipore.com ); rabbit anti-human α-fetoprotein and mouse anti-human CD31 (both from Dako, Glostrup, Denmark, http://www.dako.com ); goat anti-BMP4 and rabbit anti-c-Kit (Santa Cruz Biotechnology, Santa Cruz, CA, http://www.scbt.com ); goat anti-mouse Sca-1/Ly6 (both from R&D Systems, Minneapolis, MN, http://www.rndsystems.com ); rabbit anti-CD133, mouse anti-Nanog, and mouse anti-Perilipin (all three from Cell Signaling Technology, Beverly, MA, http://www.cellsignal.com ); mouse anti-human Nestin (Neuromics Antibodies, Edina, MN, http://www.neuromics.com ); rabbit anti-Islet-1 (Abcam, Cambridge, U.K., http://www.abcam.com ); mouse anti-human Nanog (all from Sigma-Aldrich); mouse anti-mouse Oct3/4 and rabbit anti-mouse SOX2 (both from StemCell Technologies, Vancouver, BC, Canada, http://www.stemcell.com ); goat anti-human Oct3/4 and mouse anti-human/mouse SSEA-1 (both from R&D Systems); mouse anti-TRA-1-60 (Invitrogen, Carlsbad, CA, http://www.invitrogen.com ); rabbit anti-Klf4 and rabbit anti-c-Myc (both from Abgent, San Diego, CA, http://www.abgent.com ); and rabbit anti-VE-Cadherin, rabbit anti-Troponin I, and rabbit anti-SOX9 (all three from Santa Cruz Biotechnology).

Techniques: Expressing, Marker, Reverse Transcription, Polymerase Chain Reaction, Isolation, Comparison, Real-time Polymerase Chain Reaction, Microscopy

Expression of pluripotency markers in hDFAT cells. (A): Expression of Oct3/4, SOX2, Nanog, c-Myc, Klf4, and c-Kit was detected in hDFAT cells by reverse transcription polymerase chain reaction (PCR) on day 3. Isolated human adipocytes and human embryonic stem cells were used as negative and positive controls, respectively, and human adipose stromal cells are shown for comparison. β-Actin was used as RNA control. (B): Expression of Oct3/4, SOX2, c-Myc, Klf4, and Nanog decreased between day 3 and day 15 in hDFAT cells as determined by real-time PCR. hDFAT cells after P1 were included for comparison. Asterisks indicate a statistically significant difference as compared with day 0: ∗, p < .05; ∗∗, p < .01; ∗∗∗, p < .001; Tukey’s test. (C): Characteristic hDFAT cell clusters formed spontaneously in 7–10 days in hDFAT cell cultures, as visualized by reverse-phase microscopy (BF). Images show the immunolocalization of Oct3/4 and SOX2, SSEA-3 and SSEA-4, Nanog and Klf4, c-Myc and Sca1, and CD133 and c-Kit in cell clusters. DAPI (blue) was used to visualize nuclei. Abbreviations: BF, bright field; DAPI, 4′,6-diamidino-2-phenylindole; hAdipo, human adipocytes; hASC, human adipose stromal cells; hDFAT, human dedifferentiated fat; hES, human embryonic stem cells; P1, passage 1; SOX2, sex-determining region Y-box 2; SSEA, stage-specific embryonic antigen.

Journal: Stem Cells Translational Medicine

Article Title: Pluripotent Stem Cells Derived From Mouse and Human White Mature Adipocytes

doi: 10.5966/sctm.2013-0107

Figure Lengend Snippet: Expression of pluripotency markers in hDFAT cells. (A): Expression of Oct3/4, SOX2, Nanog, c-Myc, Klf4, and c-Kit was detected in hDFAT cells by reverse transcription polymerase chain reaction (PCR) on day 3. Isolated human adipocytes and human embryonic stem cells were used as negative and positive controls, respectively, and human adipose stromal cells are shown for comparison. β-Actin was used as RNA control. (B): Expression of Oct3/4, SOX2, c-Myc, Klf4, and Nanog decreased between day 3 and day 15 in hDFAT cells as determined by real-time PCR. hDFAT cells after P1 were included for comparison. Asterisks indicate a statistically significant difference as compared with day 0: ∗, p < .05; ∗∗, p < .01; ∗∗∗, p < .001; Tukey’s test. (C): Characteristic hDFAT cell clusters formed spontaneously in 7–10 days in hDFAT cell cultures, as visualized by reverse-phase microscopy (BF). Images show the immunolocalization of Oct3/4 and SOX2, SSEA-3 and SSEA-4, Nanog and Klf4, c-Myc and Sca1, and CD133 and c-Kit in cell clusters. DAPI (blue) was used to visualize nuclei. Abbreviations: BF, bright field; DAPI, 4′,6-diamidino-2-phenylindole; hAdipo, human adipocytes; hASC, human adipose stromal cells; hDFAT, human dedifferentiated fat; hES, human embryonic stem cells; P1, passage 1; SOX2, sex-determining region Y-box 2; SSEA, stage-specific embryonic antigen.

Article Snippet: We used the following antibodies for immunostaining: rat anti-stage-specific embryonic antigen (SSEA)-3, mouse anti-SSEA-4, rabbit anti-Neurofilament-66, and mouse anti-human mitochondria (all from Millipore/Chemicon, Billerica, MA, http://www.millipore.com ); rabbit anti-human α-fetoprotein and mouse anti-human CD31 (both from Dako, Glostrup, Denmark, http://www.dako.com ); goat anti-BMP4 and rabbit anti-c-Kit (Santa Cruz Biotechnology, Santa Cruz, CA, http://www.scbt.com ); goat anti-mouse Sca-1/Ly6 (both from R&D Systems, Minneapolis, MN, http://www.rndsystems.com ); rabbit anti-CD133, mouse anti-Nanog, and mouse anti-Perilipin (all three from Cell Signaling Technology, Beverly, MA, http://www.cellsignal.com ); mouse anti-human Nestin (Neuromics Antibodies, Edina, MN, http://www.neuromics.com ); rabbit anti-Islet-1 (Abcam, Cambridge, U.K., http://www.abcam.com ); mouse anti-human Nanog (all from Sigma-Aldrich); mouse anti-mouse Oct3/4 and rabbit anti-mouse SOX2 (both from StemCell Technologies, Vancouver, BC, Canada, http://www.stemcell.com ); goat anti-human Oct3/4 and mouse anti-human/mouse SSEA-1 (both from R&D Systems); mouse anti-TRA-1-60 (Invitrogen, Carlsbad, CA, http://www.invitrogen.com ); rabbit anti-Klf4 and rabbit anti-c-Myc (both from Abgent, San Diego, CA, http://www.abgent.com ); and rabbit anti-VE-Cadherin, rabbit anti-Troponin I, and rabbit anti-SOX9 (all three from Santa Cruz Biotechnology).

Techniques: Expressing, Reverse Transcription, Polymerase Chain Reaction, Isolation, Comparison, Real-time Polymerase Chain Reaction, Microscopy

Expression of pluripotency markers in hDFAT cells. (A): Immunolocalization of Oct3/4, SOX2, SSEA-3, Nanog, Klf4, c-Myc, c-Kit, and BMP4 in single hDFAT cells after dissociation of clusters on days 7–8. DAPI (blue) was used to visualize nuclei. (B): hDFAT cells and hASCs positive for CD14, CD105, stage-specific embryonic antigen-3, and CD34 as determined by fluorescence-activated cell sorting on day 5. (C): hDFAT cells and hASCs stained for alkaline phosphatase activity on day 7. Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; hASC, human adipose stromal cell; hDFAT, human dedifferentiated fat; hES, human embryonic stem; SOX2, sex-determining region Y-box 2; SSEA, stage-specific embryonic antigen.

Journal: Stem Cells Translational Medicine

Article Title: Pluripotent Stem Cells Derived From Mouse and Human White Mature Adipocytes

doi: 10.5966/sctm.2013-0107

Figure Lengend Snippet: Expression of pluripotency markers in hDFAT cells. (A): Immunolocalization of Oct3/4, SOX2, SSEA-3, Nanog, Klf4, c-Myc, c-Kit, and BMP4 in single hDFAT cells after dissociation of clusters on days 7–8. DAPI (blue) was used to visualize nuclei. (B): hDFAT cells and hASCs positive for CD14, CD105, stage-specific embryonic antigen-3, and CD34 as determined by fluorescence-activated cell sorting on day 5. (C): hDFAT cells and hASCs stained for alkaline phosphatase activity on day 7. Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; hASC, human adipose stromal cell; hDFAT, human dedifferentiated fat; hES, human embryonic stem; SOX2, sex-determining region Y-box 2; SSEA, stage-specific embryonic antigen.

Article Snippet: We used the following antibodies for immunostaining: rat anti-stage-specific embryonic antigen (SSEA)-3, mouse anti-SSEA-4, rabbit anti-Neurofilament-66, and mouse anti-human mitochondria (all from Millipore/Chemicon, Billerica, MA, http://www.millipore.com ); rabbit anti-human α-fetoprotein and mouse anti-human CD31 (both from Dako, Glostrup, Denmark, http://www.dako.com ); goat anti-BMP4 and rabbit anti-c-Kit (Santa Cruz Biotechnology, Santa Cruz, CA, http://www.scbt.com ); goat anti-mouse Sca-1/Ly6 (both from R&D Systems, Minneapolis, MN, http://www.rndsystems.com ); rabbit anti-CD133, mouse anti-Nanog, and mouse anti-Perilipin (all three from Cell Signaling Technology, Beverly, MA, http://www.cellsignal.com ); mouse anti-human Nestin (Neuromics Antibodies, Edina, MN, http://www.neuromics.com ); rabbit anti-Islet-1 (Abcam, Cambridge, U.K., http://www.abcam.com ); mouse anti-human Nanog (all from Sigma-Aldrich); mouse anti-mouse Oct3/4 and rabbit anti-mouse SOX2 (both from StemCell Technologies, Vancouver, BC, Canada, http://www.stemcell.com ); goat anti-human Oct3/4 and mouse anti-human/mouse SSEA-1 (both from R&D Systems); mouse anti-TRA-1-60 (Invitrogen, Carlsbad, CA, http://www.invitrogen.com ); rabbit anti-Klf4 and rabbit anti-c-Myc (both from Abgent, San Diego, CA, http://www.abgent.com ); and rabbit anti-VE-Cadherin, rabbit anti-Troponin I, and rabbit anti-SOX9 (all three from Santa Cruz Biotechnology).

Techniques: Expressing, Fluorescence, FACS, Staining, Activity Assay

Expression of mulitpotent markers in hDFAT cells collected on separate days. (A): Schematic drawing of experiment in which hDFAT cells were collected from the isolated adipocytes for 4 days (days 1–4) or for 24 hours on days 2, 3, and 5. (B): Expression of Oct3/4, SOX2, Nanog, c-Myc, and Klf4 in hDFAT cells prepared by ceiling culture, or collected from floating adipocytes, as described above, as determined by real-time polymerase chain reaction. ∗, p < .05; ∗∗, p < .01; Tukey’s test. (C): Expression of SSEA-3, SSEA-4, CD105, and CD14 in hDFAT cells collected, as described above, as determined by FACS. Abbreviations: hDFAT, human dedifferentiated fat; SOX2, sex-determining region Y-box 2; SSEA, stage-specific embryonic antigen.

Journal: Stem Cells Translational Medicine

Article Title: Pluripotent Stem Cells Derived From Mouse and Human White Mature Adipocytes

doi: 10.5966/sctm.2013-0107

Figure Lengend Snippet: Expression of mulitpotent markers in hDFAT cells collected on separate days. (A): Schematic drawing of experiment in which hDFAT cells were collected from the isolated adipocytes for 4 days (days 1–4) or for 24 hours on days 2, 3, and 5. (B): Expression of Oct3/4, SOX2, Nanog, c-Myc, and Klf4 in hDFAT cells prepared by ceiling culture, or collected from floating adipocytes, as described above, as determined by real-time polymerase chain reaction. ∗, p < .05; ∗∗, p < .01; Tukey’s test. (C): Expression of SSEA-3, SSEA-4, CD105, and CD14 in hDFAT cells collected, as described above, as determined by FACS. Abbreviations: hDFAT, human dedifferentiated fat; SOX2, sex-determining region Y-box 2; SSEA, stage-specific embryonic antigen.

Article Snippet: We used the following antibodies for immunostaining: rat anti-stage-specific embryonic antigen (SSEA)-3, mouse anti-SSEA-4, rabbit anti-Neurofilament-66, and mouse anti-human mitochondria (all from Millipore/Chemicon, Billerica, MA, http://www.millipore.com ); rabbit anti-human α-fetoprotein and mouse anti-human CD31 (both from Dako, Glostrup, Denmark, http://www.dako.com ); goat anti-BMP4 and rabbit anti-c-Kit (Santa Cruz Biotechnology, Santa Cruz, CA, http://www.scbt.com ); goat anti-mouse Sca-1/Ly6 (both from R&D Systems, Minneapolis, MN, http://www.rndsystems.com ); rabbit anti-CD133, mouse anti-Nanog, and mouse anti-Perilipin (all three from Cell Signaling Technology, Beverly, MA, http://www.cellsignal.com ); mouse anti-human Nestin (Neuromics Antibodies, Edina, MN, http://www.neuromics.com ); rabbit anti-Islet-1 (Abcam, Cambridge, U.K., http://www.abcam.com ); mouse anti-human Nanog (all from Sigma-Aldrich); mouse anti-mouse Oct3/4 and rabbit anti-mouse SOX2 (both from StemCell Technologies, Vancouver, BC, Canada, http://www.stemcell.com ); goat anti-human Oct3/4 and mouse anti-human/mouse SSEA-1 (both from R&D Systems); mouse anti-TRA-1-60 (Invitrogen, Carlsbad, CA, http://www.invitrogen.com ); rabbit anti-Klf4 and rabbit anti-c-Myc (both from Abgent, San Diego, CA, http://www.abgent.com ); and rabbit anti-VE-Cadherin, rabbit anti-Troponin I, and rabbit anti-SOX9 (all three from Santa Cruz Biotechnology).

Techniques: Expressing, Isolation, Real-time Polymerase Chain Reaction

Immunological validation of ubiquitin and S100P. ( A ) For ubiquitin, four BC and corresponding AT extracts were analysed by immunoblotting, indicating relative upregulation of ubiquitin in some breast cancer patients. β -Actin is shown as a loading control. ( B ) Densitometric analysis of ubiquitin western blots of eight sample pairs. Box plot shows median and upper and lower quartiles; lines show maximum and minimum values. P =0.017, Wilcoxon signed-rank test. ( C ) Mass spectrometry (MS) spectra of proteins bound to immobilised mouse anti-ubiquitin antibody. Samples were (i) patient 1 normal tissue, (ii) patient 1 cancer tissue, (iii) patient 2 normal tissue, (iv) patient 2 cancer tissue, (v) recombinant His-tagged ubiquitin, and (vi) patient 2 cancer tissue, mouse IgG control. Arrow indicates the mass of monomeric ubiquitin, m/z 8558. N=normal tissue; C=cancer tissue. ( D ) For S100P, four BC and corresponding AT extracts were analysed by immunoblotting, indicating relative upregulation of S100P in some breast cancer patients. β -Actin is shown as a loading control. ( E ) Densitometric analysis of S100P Western blots of 8 sample pairs. Box plot shows median and upper and lower quartiles; lines show maximum and minimum values. P =0.012, Wilcoxon signed-rank test. ( F ) Mass spectrometry spectra of proteins bound to immobilised rabbit anti-S100P antibody. Samples were (i) patient 3 normal tissue, (ii) patient 3 cancer tissue, (iii) patient 4 normal tissue, (iv) patient 4 cancer tissue, (v) recombinant His-tagged S100P, and (vi) patient 4 cancer tissue, rabbit IgG control. Arrow indicates the mass of the S100P form of m/z 9226. N=normal tissue; C=cancer tissue.

Journal: British Journal of Cancer

Article Title: Tissue biomarkers of breast cancer and their association with conventional pathologic features

doi: 10.1038/bjc.2012.552

Figure Lengend Snippet: Immunological validation of ubiquitin and S100P. ( A ) For ubiquitin, four BC and corresponding AT extracts were analysed by immunoblotting, indicating relative upregulation of ubiquitin in some breast cancer patients. β -Actin is shown as a loading control. ( B ) Densitometric analysis of ubiquitin western blots of eight sample pairs. Box plot shows median and upper and lower quartiles; lines show maximum and minimum values. P =0.017, Wilcoxon signed-rank test. ( C ) Mass spectrometry (MS) spectra of proteins bound to immobilised mouse anti-ubiquitin antibody. Samples were (i) patient 1 normal tissue, (ii) patient 1 cancer tissue, (iii) patient 2 normal tissue, (iv) patient 2 cancer tissue, (v) recombinant His-tagged ubiquitin, and (vi) patient 2 cancer tissue, mouse IgG control. Arrow indicates the mass of monomeric ubiquitin, m/z 8558. N=normal tissue; C=cancer tissue. ( D ) For S100P, four BC and corresponding AT extracts were analysed by immunoblotting, indicating relative upregulation of S100P in some breast cancer patients. β -Actin is shown as a loading control. ( E ) Densitometric analysis of S100P Western blots of 8 sample pairs. Box plot shows median and upper and lower quartiles; lines show maximum and minimum values. P =0.012, Wilcoxon signed-rank test. ( F ) Mass spectrometry spectra of proteins bound to immobilised rabbit anti-S100P antibody. Samples were (i) patient 3 normal tissue, (ii) patient 3 cancer tissue, (iii) patient 4 normal tissue, (iv) patient 4 cancer tissue, (v) recombinant His-tagged S100P, and (vi) patient 4 cancer tissue, rabbit IgG control. Arrow indicates the mass of the S100P form of m/z 9226. N=normal tissue; C=cancer tissue.

Article Snippet: To confirm the identity of the m/z 8558 protein peak by protein chip immunocapture, pre-activated RS100 protein chips (Bio-Rad) were pre-coupled with 2 μ g of monoclonal anti-human ubiquitin antibody (R&D) in 50 mℳ NaHCO 3 buffer (pH 9.2) at 4 °C.

Techniques: Western Blot, Mass Spectrometry, Recombinant

Association of two protein markers and their combination with tumour histopathologic variables

Journal: British Journal of Cancer

Article Title: Tissue biomarkers of breast cancer and their association with conventional pathologic features

doi: 10.1038/bjc.2012.552

Figure Lengend Snippet: Association of two protein markers and their combination with tumour histopathologic variables

Article Snippet: To confirm the identity of the m/z 8558 protein peak by protein chip immunocapture, pre-activated RS100 protein chips (Bio-Rad) were pre-coupled with 2 μ g of monoclonal anti-human ubiquitin antibody (R&D) in 50 mℳ NaHCO 3 buffer (pH 9.2) at 4 °C.

Techniques: